In vitro/Ex vivo Respirometry

The Agilent Seahorse XFe24 Analyzer measures real-time cellular metabolism by quantifying oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) in live cells or tissue samples. These parameters reflect mitochondrial respiration and glycolysis, respectively.

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The Seahorse XFe24 can be used to:

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• Characterize mitochondrial function (e.g., basal respiration, ATP production, maximal respiratory capacity).

• Assess glycolytic activity and capacity under various conditions.

• Investigate how nutrients, hormones, or drugs shift metabolic pathways in isolated cells (e.g., hepatocytes, adipocytes, muscle cells) or even ex vivo tissues like muscle fibers or islets.

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It's a powerful tool for dissecting cellular bioenergetics and understanding how metabolic flexibility or dysfunction underlies disease phenotypes.

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For more information, contact Prof. Roland Stimson (Roland.Stimson@ed.ac.uk)