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Cellular respirometry

Agilent Seahorse XFe24

Agilent Seahorse analyser, from the original EdMPF website

The Seahorse XFe24 measures oxygen consumption and extracellular acidification in live cells or suitable ex vivo preparations. It complements whole animal studies by examining metabolic responses at the cellular level.

  • Live cells and suitable tissue preparations
  • Oxygen consumption and extracellular acidification
Discuss your study

Roland.Stimson@ed.ac.uk

What we measure

  • Oxygen consumption rate (OCR)
  • Extracellular acidification rate (ECAR)
  • Mitochondrial respiration parameters with appropriate assay design
  • Responses to substrates and pharmacological interventions

Planning your study

Discuss sample suitability, preparation, plating, assay optimisation, controls and normalisation with the platform contact before planning the experiment.

Study planning and access